Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p081 | (1) | WCRB2014

Establishment of efficient system in the DNA microinjection into porcine in vitro embryos

Malaweera Don Buddika Oshadi , Ramachandra Sisitha , Kim Ghangyong , Kim Seokjoong , Cho Jongki

To investigate the efficient laboratory techniques for the injection of DNA, pEGFP-N1 commercial plasmid were microinjected into porcine parthenogenetic and IVF embryos to explore the injection time, volume and concentration, for the efficient blastocyst production. In experiment 1, to investigate injection time, compared four different time durations (2, 4, 6 and 8 h) after post activation and 6 h of co-incubation with sperms. There were no significant difference (P&...